<p> <span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">TMB One Component Membrane Substrate为沉淀性底物，具有低本底、稳定、灵敏的特点，用于Westernblot、Microarray等应用。</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <strong style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">目录号</strong><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;"> Cat no: TMMS-0125/0250/0500/1000</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <strong style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">包装规格</strong><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;"> Package size: 125mL/0250mL/500mL/1000mL</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <strong style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">pH 值</strong><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;"> pH value: 4.5&plusmn;0.5</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <strong style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">防腐剂</strong><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;"> Biozide: 0.0002%（w/v）异噻唑啉酮</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <strong style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">保质期</strong><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;"> Shelf life: 3 years</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <strong style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">储 存</strong><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;"> Storage: 2-8℃</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <strong style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">应 用</strong><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;"> Application: </span><strong style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">Westernblot、Northernblot、Microarray</strong><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">、</span><strong style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">ELISpot</strong><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">等用途。</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <strong style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">用法说明</strong><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;"> Instruction:</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">Durastab</span><span style="padding: 0px; margin: 0px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei";">TM</span><span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;"> TMB One Component Membrane Substrate 为即用型底物，平衡至反应温度后使用。</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">Westernblot显色步骤：</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">1. 洗涤，HRP酶标孵育后，用PBST/TBST washing buffer洗3次；</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">2. 显色，加入1mL 底物，在20-37℃孵育一定时间，检测条带显色为蓝色；</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">3. 终止：用1.5mL去离子水洗涤2-3次终止显色；</span><br data-filtered="filtered" style="padding: 0px; margin: 0px; color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;" /> <span style="color: rgb(106, 106, 106); font-family: Arial, "Microsoft YaHei"; font-size: 14px;">4. 读数：肉眼判读或仪器拍照分析灰度值；</span></p>